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neurite outgrowth analysis application module of metamorph software  (MetaMorph Inc)
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a Morphology of neurites derived from NSC34 neural cells was observed under microscopy. Five experimental groups were categorized: (1) cells treated with pCS2 transfection, (2) pCS2 transfection plus Pgk1 incubation (pCS2+Pgk1), (3) pCS2-Eno1 transfection plus Pgk1 incubation (pCS2-Eno1+Pgk1), (4) pCS2-Eno2 transfection plus Pgk1 incubation (pCS2-Eno2+Pgk1), and (5) pCS2-Eno3 transfection plus Pgk1 incubation (pCS2-Eno3+Pgk1). Scale bar, 100 μm. b <t>Neurite</t> <t>Outgrowth</t> <t>Analysis</t> <t>Application</t> <t>Module</t> of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths. Cell body was marked in white, while neurite was marked in red. c , d Statistical analysis: c percentage of neurite-bearing cells among examined cells and d average neurite length of each group, as indicated. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). e Western blot analysis. NSC34 cells incubated either in the presence (+) or absence (−) of Pgk1 and transfected either pCS2-Eno1-, -Eno2-, or -Eno3-Myc, followed by analyzing the levels of phosphorylated Cofilin at S3 (p-Cofilin) and total Cofilin. The α-tubulin served as an internal control. The change (in fold) of relative intensity of each examined protein against α-tubulin of each group compared to that of the pCS2-control group relative to each internal control set as 1 was presented under each lane. Data were averaged from three independent experiments. f Statistical analysis. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). The same protein extracts from each experiment were loaded onto separate western blots and probed for individual proteins, in parallel.
Neurite Outgrowth Analysis Application Module Of Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurite+outgrowth+module/neurite+outgrowth+module+of+metamorph+software/pmc10427645-111-5-11
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antibody dependant neurite outgrowth modulation response  (Inserm Transfert)
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a Morphology of neurites derived from NSC34 neural cells was observed under microscopy. Five experimental groups were categorized: (1) cells treated with pCS2 transfection, (2) pCS2 transfection plus Pgk1 incubation (pCS2+Pgk1), (3) pCS2-Eno1 transfection plus Pgk1 incubation (pCS2-Eno1+Pgk1), (4) pCS2-Eno2 transfection plus Pgk1 incubation (pCS2-Eno2+Pgk1), and (5) pCS2-Eno3 transfection plus Pgk1 incubation (pCS2-Eno3+Pgk1). Scale bar, 100 μm. b <t>Neurite</t> <t>Outgrowth</t> <t>Analysis</t> <t>Application</t> <t>Module</t> of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths. Cell body was marked in white, while neurite was marked in red. c , d Statistical analysis: c percentage of neurite-bearing cells among examined cells and d average neurite length of each group, as indicated. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). e Western blot analysis. NSC34 cells incubated either in the presence (+) or absence (−) of Pgk1 and transfected either pCS2-Eno1-, -Eno2-, or -Eno3-Myc, followed by analyzing the levels of phosphorylated Cofilin at S3 (p-Cofilin) and total Cofilin. The α-tubulin served as an internal control. The change (in fold) of relative intensity of each examined protein against α-tubulin of each group compared to that of the pCS2-control group relative to each internal control set as 1 was presented under each lane. Data were averaged from three independent experiments. f Statistical analysis. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). The same protein extracts from each experiment were loaded onto separate western blots and probed for individual proteins, in parallel.
Antibody Dependant Neurite Outgrowth Modulation Response, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neurite outgrowth module  (MetaMorph Inc)
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MetaMorph Inc neurite outgrowth module
a Morphology of neurites derived from NSC34 neural cells was observed under microscopy. Five experimental groups were categorized: (1) cells treated with pCS2 transfection, (2) pCS2 transfection plus Pgk1 incubation (pCS2+Pgk1), (3) pCS2-Eno1 transfection plus Pgk1 incubation (pCS2-Eno1+Pgk1), (4) pCS2-Eno2 transfection plus Pgk1 incubation (pCS2-Eno2+Pgk1), and (5) pCS2-Eno3 transfection plus Pgk1 incubation (pCS2-Eno3+Pgk1). Scale bar, 100 μm. b <t>Neurite</t> <t>Outgrowth</t> <t>Analysis</t> <t>Application</t> <t>Module</t> of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths. Cell body was marked in white, while neurite was marked in red. c , d Statistical analysis: c percentage of neurite-bearing cells among examined cells and d average neurite length of each group, as indicated. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). e Western blot analysis. NSC34 cells incubated either in the presence (+) or absence (−) of Pgk1 and transfected either pCS2-Eno1-, -Eno2-, or -Eno3-Myc, followed by analyzing the levels of phosphorylated Cofilin at S3 (p-Cofilin) and total Cofilin. The α-tubulin served as an internal control. The change (in fold) of relative intensity of each examined protein against α-tubulin of each group compared to that of the pCS2-control group relative to each internal control set as 1 was presented under each lane. Data were averaged from three independent experiments. f Statistical analysis. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). The same protein extracts from each experiment were loaded onto separate western blots and probed for individual proteins, in parallel.
Neurite Outgrowth Module, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the neurite outgrowth module  (MetaMorph Inc)
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MetaMorph Inc the neurite outgrowth module
a Morphology of neurites derived from NSC34 neural cells was observed under microscopy. Five experimental groups were categorized: (1) cells treated with pCS2 transfection, (2) pCS2 transfection plus Pgk1 incubation (pCS2+Pgk1), (3) pCS2-Eno1 transfection plus Pgk1 incubation (pCS2-Eno1+Pgk1), (4) pCS2-Eno2 transfection plus Pgk1 incubation (pCS2-Eno2+Pgk1), and (5) pCS2-Eno3 transfection plus Pgk1 incubation (pCS2-Eno3+Pgk1). Scale bar, 100 μm. b <t>Neurite</t> <t>Outgrowth</t> <t>Analysis</t> <t>Application</t> <t>Module</t> of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths. Cell body was marked in white, while neurite was marked in red. c , d Statistical analysis: c percentage of neurite-bearing cells among examined cells and d average neurite length of each group, as indicated. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). e Western blot analysis. NSC34 cells incubated either in the presence (+) or absence (−) of Pgk1 and transfected either pCS2-Eno1-, -Eno2-, or -Eno3-Myc, followed by analyzing the levels of phosphorylated Cofilin at S3 (p-Cofilin) and total Cofilin. The α-tubulin served as an internal control. The change (in fold) of relative intensity of each examined protein against α-tubulin of each group compared to that of the pCS2-control group relative to each internal control set as 1 was presented under each lane. Data were averaged from three independent experiments. f Statistical analysis. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). The same protein extracts from each experiment were loaded onto separate western blots and probed for individual proteins, in parallel.
The Neurite Outgrowth Module, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neurite+outgrowth+module/neurite+outgrowth+application/pmc04688065-191-12-16
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a Morphology of neurites derived from NSC34 neural cells was observed under microscopy. Five experimental groups were categorized: (1) cells treated with pCS2 transfection, (2) pCS2 transfection plus Pgk1 incubation (pCS2+Pgk1), (3) pCS2-Eno1 transfection plus Pgk1 incubation (pCS2-Eno1+Pgk1), (4) pCS2-Eno2 transfection plus Pgk1 incubation (pCS2-Eno2+Pgk1), and (5) pCS2-Eno3 transfection plus Pgk1 incubation (pCS2-Eno3+Pgk1). Scale bar, 100 μm. b Neurite Outgrowth Analysis Application Module of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths. Cell body was marked in white, while neurite was marked in red. c , d Statistical analysis: c percentage of neurite-bearing cells among examined cells and d average neurite length of each group, as indicated. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). e Western blot analysis. NSC34 cells incubated either in the presence (+) or absence (−) of Pgk1 and transfected either pCS2-Eno1-, -Eno2-, or -Eno3-Myc, followed by analyzing the levels of phosphorylated Cofilin at S3 (p-Cofilin) and total Cofilin. The α-tubulin served as an internal control. The change (in fold) of relative intensity of each examined protein against α-tubulin of each group compared to that of the pCS2-control group relative to each internal control set as 1 was presented under each lane. Data were averaged from three independent experiments. f Statistical analysis. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). The same protein extracts from each experiment were loaded onto separate western blots and probed for individual proteins, in parallel.

Journal: Communications Biology

Article Title: Extracellular Pgk1 interacts neural membrane protein enolase-2 to improve the neurite outgrowth of motor neurons

doi: 10.1038/s42003-023-05223-0

Figure Lengend Snippet: a Morphology of neurites derived from NSC34 neural cells was observed under microscopy. Five experimental groups were categorized: (1) cells treated with pCS2 transfection, (2) pCS2 transfection plus Pgk1 incubation (pCS2+Pgk1), (3) pCS2-Eno1 transfection plus Pgk1 incubation (pCS2-Eno1+Pgk1), (4) pCS2-Eno2 transfection plus Pgk1 incubation (pCS2-Eno2+Pgk1), and (5) pCS2-Eno3 transfection plus Pgk1 incubation (pCS2-Eno3+Pgk1). Scale bar, 100 μm. b Neurite Outgrowth Analysis Application Module of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths. Cell body was marked in white, while neurite was marked in red. c , d Statistical analysis: c percentage of neurite-bearing cells among examined cells and d average neurite length of each group, as indicated. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). e Western blot analysis. NSC34 cells incubated either in the presence (+) or absence (−) of Pgk1 and transfected either pCS2-Eno1-, -Eno2-, or -Eno3-Myc, followed by analyzing the levels of phosphorylated Cofilin at S3 (p-Cofilin) and total Cofilin. The α-tubulin served as an internal control. The change (in fold) of relative intensity of each examined protein against α-tubulin of each group compared to that of the pCS2-control group relative to each internal control set as 1 was presented under each lane. Data were averaged from three independent experiments. f Statistical analysis. Data were averaged from three independent experiments and presented as mean ± SD ( n = 3). One-way ANOVA, followed by Tukey’s multiple comparison test, was used to perform statistical analysis (** P < 0.005 and NS, not significant, P > 0.05). The same protein extracts from each experiment were loaded onto separate western blots and probed for individual proteins, in parallel.

Article Snippet: Scale bar, 100 μm. b Neurite Outgrowth Analysis Application Module of MetaMorph software was used to count neurite-bearing cells and measure neurite lengths.

Techniques: Derivative Assay, Microscopy, Transfection, Incubation, Software, Comparison, Western Blot, Control